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KMID : 0903519930360050370
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1993 Volume.36 No. 5 p.370 ~ p.375
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Extraction and fractionation of proteins having both chitinase and β - 1,3 - glucanase activities from rice leaves
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Abstract
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Five electrophoretic bands of crude enzyme extracted from rice leaves were found to possess both chitinase and β-1,3-glucanase activities. These chitinase/β-1,3-glucanases were resolved into acidic and basic fractions of protein by DEAF-cellulose and chitin affinity column chromatography. The optimal pH and temperature for β-1,3-glucanase activity of two fractions were in the same extent as pH 5 and 60℃, whereas those for chitinase activity differed from one another; pH 3 and 60℃ for the acidic and pH 4 and 50℃ for the basic fraction, respectively. In addition, lysozyme activity was found in both fractions.
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